The arrival of the Ark of the Covenant at the Israelite camp creates a sudden and dramatic shift in the atmosphere of the battlefield. The Philistines are immediately gripped by terror as they realize the rules of engagement have fundamentally changed. Their profound fear arises from the belief that God, who dwells upon the Ark, has entered the camp to fight against them [ביאור שטיינזלץ] [מלבי״ם].
Their cry of despair reveals a deep anxiety about this new reality. They understand that while they have consistently defeated the Israelites up to this point, those previous victories were won against mere flesh and blood. Now, the dynamic has shifted entirely, and they find themselves standing directly against the God who saves Israel [מלבי״ם].
Adding to their panic is the completely unprecedented nature of the situation. In all their past wars with the Hebrews, the Ark had never once been brought out to the front lines [ביאור שטיינזלץ] [מצודת דוד]. Its sudden presence marks a historic first, an absolute break from anything they had experienced in the recent past or in any of the days leading up to this moment [מצודת ציון].